Key result
Weighted gene co-expression network analysis identified nine crucial hub genes, including LRRC39 (AUC 0.982) and COQ10A (AUC 0.959), and immune cell infiltration patterns underlying sepsis-induced cardiomyopathy.
Why the study?
The overall situation of gene co-expression and how it affects the pathological process of sepsis-induced cardiomyopathy remained obscure despite known mechanisms.
Observational (n=31)
LRRC39 and COQ10A merit prospective validation as biomarkers; hypothesis-generating for sepsis-induced cardiomyopathy and should not yet change practice.
Sepsis-induced cardiomyopathy (SIC), with a possibly reversible cardiac dysfunction, is a potential complication of septic shock. Despite quite a few mechanisms including the inflammatory mediator, exosomes, and mitochondrial dysfunction, having been confirmed in the existing research studies we still find it obscure about the overall situation of gene co-expression that how they can affect the pathological process of SIC. Thus, we intended to find out the crucial hub genes, biological signaling pathways, and infiltration of immunocytes underlying SIC. It was weighted gene co-expression network analysis that worked as our major method on the ground of the gene expression profiles: hearts of those who died from sepsis were compared to hearts donated by non-failing humans which could not be transplanted for technical reasons (GSE79962). The top 25 percent of variant genes were abstracted to identify 10 co-expression modules. In these modules, brown and green modules showed the strongest negative and positive correlation with SIC, which were primarily enriched in the bioenergy metabolism, immunoreaction, and cell death. Next, nine genes ( LRRC39 , COQ10A , FSD2 , PPP1R3A , TNFRSF11B , IL1RAP , DGKD , POR , and THBS1 ) including two downregulated and seven upregulated genes which were chosen as hub genes that meant the expressive level of which was higher than the counterparts in control groups. Then, the gene set enrichment analysis (GSEA) demonstrated a close relationship of hub genes to the cardiac metabolism and the necroptosis and apoptosis of cells in SIC. Concerning immune cells infiltration, a higher level of neutrophils and B cells native and a lower level of mast cells resting and plasma cells had been observed in patients with SIC. In general, nine candidate biomarkers were authenticated as a reliable signature for deeper exploration of basic and clinical research studies on SIC.
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Li et al. (2021) conducted an observational in Sepsis-induced cardiomyopathy (n=31). Sepsis vs. Non-failing human donors was evaluated on Identification of crucial hub genes and infiltrating immune cells. Weighted gene co-expression network analysis identified nine crucial hub genes, including LRRC39 (AUC 0.982) and COQ10A (AUC 0.959), and immune cell infiltration patterns underlying sepsis-induced cardiomyopathy.
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