What question did this study set out to answer?

To determine whether adenosine regulates skeletal muscle exercise hyperemia in synergy with prostaglandins and nitric oxide by stimulating their downstream formation.

May 12, 2009HypertensionOpen Access

Adenosine Contributes to Blood Flow Regulation in the Exercising Human Leg by Increasing Prostaglandin and Nitric Oxide Formation

Q: What is the clinical evidence from this study?

Study design: Other. Population: Healthy (n=24). Intervention: Adenosine receptor blockade, prostaglandin inhibition, and NO inhibition vs. Uninhibited state. Primary outcome: Exercise-induced increase in leg blood flow (p=<0.05).

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Key result

Adenosine receptor blockade reduced exercise-induced leg blood flow by 14% (P<0.05), and adenosine-induced vasodilation was inhibited by 66% with combined prostaglandin and NO inhibition (P<0.05).

Why the study?

Does adenosine regulate skeletal muscle hyperemia during exercise via the formation of prostaglandins and nitric oxide in healthy males?

Population

24 healthy, male subjects

Comparison

Adenosine receptor blockade, prostaglandin… vs Uninhibited exercise state and baseline resting…

Design

Other

Authors

SMStefan P. MortensenUniversity of Southern Denmark MNMichael NybergVascular Medicine PTPia ThaningHvidovre Hospital

Discussion

Member takes

Implication

Supports modest adenosine contribution to exercise hyperemia via prostaglandins and NO in healthy males; leaves open relevance to patients with cardiovascular disease.

Key Points

To determine whether adenosine regulates skeletal muscle exercise hyperemia in synergy with prostaglandins and nitric oxide by stimulating their downstream formation.
Assessed leg blood flow responses in 24 healthy male subjects during leg exercise under adenosine receptor blockade (theophylline, 399±9 mg), prostaglandin inhibition (indomethacin, 0.8–1.8 mg/min), and nitric oxide synthase inhibition (L-NMMA, 29–52 mg/min).
Measured femoral arterial and venous plasma adenosine levels via microdialysis and evaluated vasodilation during femoral arterial adenosine infusions with selective or combined pathway inhibition.
Adenosine receptor blockade attenuated the exercise-induced increase in leg blood flow by 14±1% and combined PG/NO inhibition reduced it by 29±2% (both P<0.05), whereas triple inhibition produced no additive reduction (31±5%).
Adenosine infusion increased femoral blood flow from approximately 0.3 to 2.5 L/min; this response was suppressed by prostaglandin inhibition (51±3%), NO inhibition (39±8%), and combined inhibition (66±8%, P<0.05).

Structured PICO

Does adenosine regulate skeletal muscle hyperemia during exercise via the formation of prostaglandins and nitric oxide in healthy males?

Population

24 healthy male subjects undergoing leg exercise to evaluate the role of adenosine, prostaglandins, and NO in skeletal muscle hyperemia.

Intervention

Adenosine receptor blockade (theophylline 399+/-9 mg), prostaglandin inhibition (indomethacin 0.8 to 1.8 mg/min), nitric oxide inhibition (N(G)-mono-methyl-l-arginine 29 to 52 mg/min), and femoral arterial infusion of adenosine

Comparator

Uninhibited exercise state and baseline resting state

Outcome

Leg blood flow during leg exercise and during femoral arterial infusion of adenosinesurrogate

Main Result

p-value: p=<0.05

Adenosine contributes to the regulation of skeletal muscle blood flow during exercise primarily by stimulating the synthesis of prostaglandins and nitric oxide.

Cite This Study

Mortensen et al. (2009) studied Healthy (n=24). Adenosine receptor blockade, prostaglandin inhibition, and NO inhibition vs. Uninhibited state was evaluated on Exercise-induced increase in leg blood flow (p=<0.05). Adenosine receptor blockade reduced exercise-induced leg blood flow by 14% (P<0.05), and adenosine-induced vasodilation was inhibited by 66% with combined prostaglandin and NO inhibition (P<0.05).

synapsesocial.com/papers/6a830a2d86395db871b38119 https://doi.org/10.1161/hypertensionaha.109.130880

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Implication

Supports modest adenosine contribution to exercise hyperemia via prostaglandins and NO in healthy males; leaves open relevance to patients with cardiovascular disease.

Adenosine Contributes to Blood Flow Regulation in the Exercising Human Leg by Increasing Prostaglandin and Nitric Oxide Formation

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Adenosine Contributes to Blood Flow Regulation in the Exercising Human Leg by Increasing Prostaglandin and Nitric Oxide Formation

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider