Isolation and molecular characteristics of D2 genotype of Aichivirus D in dairy cattle in China

Figures

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  Figure 1. Phylogenetic trees based on complete AiV VP0, VP3, and VP1 genes. Maximum-likelihood analysis in combination with 1,000 bootstrap replicates was used to derive a phylogenetic tree based on the complete nucleotide sequences of AiV VP0, VP3, and VP1 genes. ▲represents the AiV strains from this study.

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  Figure 2. Schematic representation of the dairy cattle AiV-D genome in this study. The length of each gene is indicated by plain numbers. The differences in length between strains are indicated by gray background numbers. Black triangles show predicted cleavage sites. And a schematic diagram of unique amino acid changes in VP0, VP3, and VP1 were marked.

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  Figure 3. Isolation and identification of dairy cattle AiV-D2. (A) Vero cell infected with AiV-D2 strain showing CPE at 72 h; (B) Mock infection negative control Vero cell; (C) IF of AiV-D2 strain; (D) IF negative control (mock infected cells); (E) and (F) TEM image of AiV-D2 strain.

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  Figure 4. Phylogenetic tree based on the complete genomes of all AiV species (A-F). Maximum-likelihood analysis in combination with 1,000 bootstrap replicates was used to derive a phylogenetic tree based on the complete genome sequences of six species AiV strains. ▲represents the AiV-D2 strain from this study.

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  Figure 5. The predicted secondary structures of the 5′-UTR of the genome of AiV-D2. Bold and gray indicate differences between two strains. The positions of the 3 stem-loops are shown. Schematic of secondary structures was predicted using Mfold software (http://www.unafold.org/mfold/applications/dna-folding-form.php) and modified using RNAviz (http://rnaviz.sourceforge.Net/).