Key result
Metagenomic sequencing of a European badger enteric specimen identified a novel picornavirus, proposed as Sakobuvirus B, sharing ~59% sequence identity with feline picornavirus.
Why the study?
Unbiased metagenomic next-generation sequencing has made it necessary to expand knowledge regarding virus diversity in wildlife and monitor potential transmission to domestic animals or humans.
Observational (n=1)
The identification of a novel picornavirus in a European badger expands the known diversity of wildlife viromes.
No immediate clinical implications; extends wildlife virome diversity and leaves open zoonotic questions.
The recent development of unbiased metagenomic next-generation sequencing has provided a richer view of the wild animal virome making it necessary to expand the knowledge about virus diversity in wildlife, as well as to monitor their potential transmission to domestic animals or humans. In the present study, by screening collections of enteric specimens from wild animals, a novel picornavirus was identified in the intestinal content of a badger (Meles meles). By enrichment with a sequence-independent single-primer amplification (SISPA) approach and deep sequencing with Oxford Nanopore Technologies (ONT) platform, the genome sequence of a novel picornavirus strain, Badger/3A-2019/ITA, was reconstructed. On comparison based on the polyprotein sequences, the virus was distantly related (58.7% and 59.7% sequence identity at the nucleotide and amino acid level, respectively) to the feline picornavirus strain FFUP1, identified in 2012 in Portugal and classified into genus Sakobovirus within the species Sakobuvirus A. Upon phylogenetic, pairwise homology, and distance analyses performed on the P1, 2Chel, 3Cpro, and 3Dpol proteins and the complete genomic sequence, the badger picornavirus may be considered a member of a new sakobuvirus species, which we propose as Sakobuvirus B.
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Palombieri et al. (2022) conducted an observational in Novel picornavirus infection (n=1). Metagenomic next-generation sequencing was evaluated on Identification and genomic characterization of a novel picornavirus. Metagenomic sequencing of a European badger enteric specimen identified a novel picornavirus, proposed as Sakobuvirus B, sharing ~59% sequence identity with feline picornavirus.
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