Anti-thrombosis Effects and Mechanisms by Xueshuantong Capsule Under Different Flow Conditions

Figures

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  Figure 1. Effects of XST on viability of HUVECs. Cells were treated with 0.9% saline water or XST for 24 h. Cell growth was measured by MTT assay. The data was presented as means ± SD, n = 5. ∗∗P < 0.01 vs. control.

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  Figure 2. Comparisons of platelets activation between groups. Representative scatter diagrams showing platelet CD62p expression (A) under 0.1 Pa flow condition, and (B) under 0.9 Pa flow condition. (C) Comparison of CD62p positive percentage between groups under different flow conditions. The percentages of CD61+ CD62p+ platelets are depicted in the upper right quadrants of the contour plots, the CD61+ CD62p- and the CD61- CD62p+ platelets are depicted in the lower right and upper left quadrants, respectively, and CD61- CD62p- platelets are depicted in the lower left quadrants. The data was presented as means ± SD, n = 3; data was analyzed by two-way ANOVA as described in Methods; analysis of simple effects.

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  Figure 3. Comparisons of platelets adhesion between groups. (A) under 0.1 Pa flow condition, and (B) under 0.9 Pa flow condition (magnification × 200). (C) Histogram demonstrating the platelets adherence on activated HUVECs under different flow conditions. The data was presented as means ± SD, n = 9; data was analyzed by two-way ANOVA as described in Methods; analysis of simple effects. ∗∗P < 0.001.

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  Figure 4. Comparisons of THP-1 adhesion between groups. (A) under 0.1 Pa flow condition, and (B) under 0.9 Pa flow condition (magnification × 200). (C) Histogram demonstrating the THP-1 cells adherence on activated HUVECs under different flow conditions. The data was presented as means ± SD, n = 9; data was analyzed by two-way ANOVA as described in Methods; analysis of simple effects. ∗∗P < 0.001.

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  Figure 5. Comparisons of VE-cadherin and Cx43 expression between groups under static condition. (A) The expression and density of VE-cadherin under static condition were analyzed by western blotting. (B) The expression and density of Cx43 under static condition were analyzed. The data was presented as means ± SD, n = 3. ∗P < 0.05 vs. model.

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  Figure 6. Comparisons of VE-cadherin and Cx43 expression between groups under 0.1 and 0.9 Pa flow conditions. (A) The expression and density of VE-cadherin under 0.1 and 0.9 Pa flow conditions were analyzed by western blotting. (B) The expression and density of Cx43 under 0.1 and 0.9 Pa flow conditions were analyzed. The data was presented as means ± SD, n = 3; data was analyzed by two-way ANOVA as described in Methods; analysis of simple effects. ∗∗P < 0.001; ∗P < 0.05.