Trichoderma harzianum isolate ThzID1 was grown in liquid culture, was formulated with alginate and polyethylene glycol 8000, and was milled into fine granules (average diameter 500 μm). Granules contained chlamydospores, conidia, and hyphal fragments. Viability of the encapsulated fungus remained high for at least 6 mo when stored at 5 C (i.e., >90% of the granules produced hyphal growth when incubated on agar); viability was reduced significantly when granules were stored at 22 C. Application of the granular formulation of T. harzianum to pea seeds reduced root rot by Aphanomyces euteiches f. sp. pisi in growth-chamber experiments and also increased plant top weights compared to noncoated seeds []
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Louise‐Marie Dandurand (1993) studied this question.