What question did this study set out to answer?

Characterize the DNA-binding properties of the Drosophila GCM protein and evaluate whether its functional domain is evolutionarily conserved in mammals.

December 10, 1996Proceedings of the National Academy of SciencesOpen Access

The gcm-motif: A novel DNA-binding motif conserved in Drosophila and mammals

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Authors

YAYasuko Akiyama THToshihiko HosoyaRicoh (Japan)APAnthony M. PooleUniversity of Auckland

Discussion

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Implication

Molecular analysis reveals a conserved GCM DNA-binding motif in Drosophila and mammals, indicating a shared transcriptional mechanism in cell fate determination.

Key Points

Characterize the DNA-binding properties of the Drosophila GCM protein and evaluate whether its functional domain is evolutionarily conserved in mammals.
Mapped the DNA-binding region of Drosophila GCM using protein truncation and sequence-specific binding assays.
Analyzed the 5' upstream regulatory region of the glial-specific repo gene for putative GCM-binding sites.
Identified and isolated homologous mammalian genes from mouse and human using sequence alignment and PCR amplification.
GCM acts as a novel DNA-binding protein with activity localized to its N-terminal 181 amino acids, targeting the specific consensus sequence (A/G)CCCGCAT.
Identified 11 GCM-binding consensus sequences in the 5' upstream region of repo, indicating direct transcriptional regulation by GCM.
Characterized homologous mouse and human proteins that share the conserved N-terminal 'gcm-motif' and exhibit corresponding DNA-binding capability.

Cite This Study

Akiyama et al. (1996) studied this question.

synapsesocial.com/papers/6a831ff386395db871b388df https://doi.org/10.1073/pnas.93.25.14912

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December 10, 1996Proceedings of the National Academy of SciencesOpen Access

The gcm-motif: A novel DNA-binding motif conserved in Drosophila and mammals

View Full Paper

Ask AI

Bookmark

Authors

YAYasuko Akiyama THToshihiko HosoyaRicoh (Japan)APAnthony M. PooleUniversity of Auckland

Discussion

Member takes

Implication

Molecular analysis reveals a conserved GCM DNA-binding motif in Drosophila and mammals, indicating a shared transcriptional mechanism in cell fate determination.

Key Points

Characterize the DNA-binding properties of the Drosophila GCM protein and evaluate whether its functional domain is evolutionarily conserved in mammals.
Mapped the DNA-binding region of Drosophila GCM using protein truncation and sequence-specific binding assays.
Analyzed the 5' upstream regulatory region of the glial-specific repo gene for putative GCM-binding sites.
Identified and isolated homologous mammalian genes from mouse and human using sequence alignment and PCR amplification.
GCM acts as a novel DNA-binding protein with activity localized to its N-terminal 181 amino acids, targeting the specific consensus sequence (A/G)CCCGCAT.
Identified 11 GCM-binding consensus sequences in the 5' upstream region of repo, indicating direct transcriptional regulation by GCM.
Characterized homologous mouse and human proteins that share the conserved N-terminal 'gcm-motif' and exhibit corresponding DNA-binding capability.

Cite This Study

Akiyama et al. (1996) studied this question.

synapsesocial.com/papers/6a831ff386395db871b388df https://doi.org/10.1073/pnas.93.25.14912

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