Concurrent Circulation of Viral Agents in Pediatric Patients Presenting with Respiratory Illness and Diarrheal Symptoms in Metropolitan Region of São Paulo, Brazil, 2021

Figures

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  Figure 1. Maximum likelihood phylogenetic tree of the partial VP1/VP2 HBoV-1 protein nucleotide sequence (658-bp fragment) generated with MEGA X software [22] of the Brazilian HBoV-1 strains detected from children with acute respiratory disease and diarrheic symptoms, Barueri, São Paulo, Brazil, 2021 (highlighted in red). HBoV prototype strains were obtained from GenBank database. HBoV assigned genotypes are indicated on the right. Accession numbers of each strain are displayed. The scale indicates the number of divergent nucleotide residues. The percentage of bootstrap values is shown at the branch node.

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  Figure 2. Phylogenetic analysis of Brazilian NoV strains detected from children with acute respiratory disease and diarrheic symptoms, Barueri, São Paulo, Brazil, 2021 (highlighted in red) alongside other selected NoV strains. Maximum-likelihood trees are presented for partial ORF 1 (A) and ORF 2 (B). Reference NoV sequences were retrieved from the GenBank database, with each strain’s accession number, isolate, country, and year indicated. Clusters identified in the present study are denoted by letters a, b, and c. The NoV G-types (ORF2) and P-types (ORF1) are specified. Statistical significance for each branch point was determined using 1000 pseudo-replicate datasets. The scale bar represents nucleotide substitutions per site, and bootstrap values are displayed as percentages at the branch nodes.

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  Figure 3. Maximum likelihood phylogenetic tree of the partial Hexon nucleotide (449-bp fragment) generated with MEGA X software [22] of the Brazilian HAdV-C6 strains detected from children with acute respiratory disease and diarrheic symptoms, Barueri, São Paulo, Brazil, 2021 (highlighted in red). HAdV prototype strains were obtained from GenBank database. HAdV assigned genotypes are indicated on the right. Accession numbers of each strain are displayed. The scale indicates the number of divergent nucleotide residues. The percentage of bootstrap values is shown at the branch node.

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  Figure 4. Maximum likelihood phylogenetic tree of the partial VP1 protein nucleotide (334-bp fragment) generated with MEGA X software [22] of the Brazilian CVA6 strains detected from children with acute respiratory disease and diarrheic symptoms, Barueri, São Paulo, Brazil, 2021 (highlighted in red). CVA6 prototype strains were obtained from GenBank database. CVA6 assigned lineages are indicated on the right. Accession numbers of each strain are displayed. The scale indicates the number of divergent nucleotide residues. The percentage of bootstrap values is shown at the branch node.

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  Figure 5. Maximum likelihood phylogenetic tree of the partial VP3/VP1 junction region (280-bp fragment) generated with MEGA X software [22] of the Brazilian PeV-A1 strain detected from children with acute respiratory disease and diarrheic symptoms, Barueri, São Paulo, Brazil, 2021 (highlighted in red). PeV-A prototype strains were obtained from GenBank database. PeV-A1 assigned subtypes are indicated on the right. Accession numbers of each strain are displayed. The scale indicates the number of divergent nucleotide residues. The percentage of bootstrap values is shown at the branch node.