A highly sensitive square-wave adsorptive anodic stripping voltammetric method was developed for the rapid and accurate determination of ribavirin in pharmaceutical formulations and human serum without the need for sample pretreatment.
May support ribavirin monitoring in serum; leaves open validation for clinical use.
A highly sensitive square-wave adsorptive anodic stripping voltammetric method is described for the determination of ribavirin in its pharmaceutical formulations and human blood serum, utilizing a carbon paste electrode. The peak current was significantly enhanced due to the strong adsorptive properties of the drug at the electrode surface. The optimal procedural parameters were: frequency of 20 Hz, scan increment of 4 mV, pulse amplitude of 25 mV, and accumulation potential of –0.5 V versus Ag/AgCl/3 M KCl in Britton–Robinson buffer at pH 8.0. The described method was successfully applied for the assay of ribavirin in its pharmaceutical formulation (Ribavirin® capsules, 200 mg ribavirin), with mean percentage recoveries of (98.3 ± 1.3)%. Furthermore, the method was successfully applied for the assay of ribavirin in spiked human blood serum without the necessity for sample pretreatment or time-consuming extraction prior to analysis. The mean percentage recovery of ribavirin in human blood serum was (98.6 ± 1.1)% (n = 5), with a limit of detection of 1 × 10–9 M (0.24 ng/mL). Due to this extremely low limit of detection, the proposed method can be applied in pharmacokinetic and pharmacodynamic studies of the drug.
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Beltagi et al. (2025) studied this question.
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