What question did this study set out to answer?

To establish an enzymatic method using collagenase and hyaluronidase for isolating intact, viable rat liver parenchymal cells and to characterize their respiratory activity.

June 1, 1968Journal of Biological ChemistryOpen Access

Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver

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Authors

RHR B HowardUniversity of Colorado Health LPLeroy A. PeschOlgahospital

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Implication

Laboratory study demonstrates sustained endogenous respiration in isolated rat liver parenchymal cells, highlighting an improved enzymatic isolation technique.

Key Points

To establish an enzymatic method using collagenase and hyaluronidase for isolating intact, viable rat liver parenchymal cells and to characterize their respiratory activity.
Digested rat liver tissue using an enzymatic preparation of collagenase and hyaluronidase to extract intact parenchymal cells.
Measured cellular structural integrity, viability, and respiration in Krebs-Ringer phosphate, glucose-free Hanks solution, and Medium 199 without added substrate.
Assessed metabolic responses over time and in the presence of calcium ions, 1% albumin, and glucose.
Yielded cell preparations with 90% to 95% structural intactness and initial viabilities ranging from 75% to 95%.
Maintained linear or near-linear rates of endogenous respiration for 2 hours, with approximately 25% of cells remaining viable and respiring after 24 hours in Medium 199.
Observed increased respiration upon the addition of calcium ions and 1% albumin, whereas glucose produced no stimulatory effect.

Cite This Study

Howard et al. (1968) studied this question.

synapsesocial.com/papers/6a83337928b8a62be9990bb2 https://doi.org/10.1016/s0021-9258(18)93383-1

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Implication

Laboratory study demonstrates sustained endogenous respiration in isolated rat liver parenchymal cells, highlighting an improved enzymatic isolation technique.

Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

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