What question did this study set out to answer?

To identify circulating glycated insulin in individuals with type 2 diabetes and quantify its in vivo biological potency and receptor-binding characteristics.

February 1, 2003DiabetesOpen Access

Demonstration of Glycated Insulin in Human Diabetic Plasma and Decreased Biological Activity Assessed by Euglycemic-Hyperinsulinemic Clamp Technique in Humans

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To identify circulating glycated insulin in individuals with type 2 diabetes and quantify its in vivo biological potency and receptor-binding characteristics.
Purified plasma from four male type 2 diabetic subjects (HbA1c 8.1 ± 0.2%) using HPLC and analyzed insulin species using electrospray ionization mass spectrometry and radioimmunoassay.
Administered synthetic monoglycated insulin to seven healthy nonobese male volunteers during a two-step euglycemic-hyperinsulinemic clamp (0.4 and 2.0 mU/kg/min) and measured CHO-T cell insulin receptor binding.
Monoglycated insulin was detected in diabetic plasma at 10.1 ± 2.3 pmol/l (approximately 9% of total insulin), exhibiting a 162.7-Da glucitol adduct compared to native insulin.
During low-dose clamp infusion (0.4 mU/kg/min), glucose infusion rates were significantly lower (P < 0.01), requiring approximately 70% more glycated insulin to achieve equivalent glucose disposal.
Maximal glucose uptake at 2.0 mU/kg/min, suppression of lipolysis and endogenous glucose production, and insulin receptor binding affinity were equivalent between glycated and native insulin.

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Implication

Experimental physiological study demonstrates impaired insulin-mediated glucose uptake by glycated insulin in healthy volunteers, indicating a postreceptor mechanism in diabetic glucose toxicity.

Cite This Study

Hunter et al. (2003) studied this question.

synapsesocial.com/papers/6a8333ec292d2964071f3107 https://doi.org/10.2337/diabetes.52.2.492

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Implication

Experimental physiological study demonstrates impaired insulin-mediated glucose uptake by glycated insulin in healthy volunteers, indicating a postreceptor mechanism in diabetic glucose toxicity.

Demonstration of Glycated Insulin in Human Diabetic Plasma and Decreased Biological Activity Assessed by Euglycemic-Hyperinsulinemic Clamp Technique in Humans

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Discussion

Implication

Cite This Study

Demonstration of Glycated Insulin in Human Diabetic Plasma and Decreased Biological Activity Assessed by Euglycemic-Hyperinsulinemic Clamp Technique in Humans

Key Points

Discussion

Implication

Cite This Study

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