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Isolated chloroplasts from Pinus silvestris have been fractionated by a combination of digitonin and Yeda‐press treatment. Different subchloroplast particles have been isolated by differential centrifugation. The spectral and photochemical properties of the different fractions have been analysed. Photosystem I was enriched in the light particles (high Chl a /Chl b ratios, low Chl/P700 ratios, high F735/F685 ratios, high photosystem I activity and enrichment of long‐wavelength absorbing Chl a ). The heavy fractions were enriched in photosystem II (low Chl a /Chl b ratios, high Chl/P700 ratios, low F735/F685 ratios and enrichment of short‐wavelength absorbing Chl a ). The molar ratio Chl/P700 was about 1.5 times greater for Pinus than for Spinacia , and Pinus had relatively less long‐wavelength absorbing Chl a compared with Spinacia .
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Gunnar Öquist (1975) studied this question.
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