A novel alveoli-on-chip platform for modeling cyclic stretch in patient-derived alveolar epithelial cells cultured from organoids

) subtypes. Dissociated lung organoids cells from three patients were cultured on the chip under conditions with or without physiological mechanical stress. Bulk RNA sequencing revealed that cyclic stretch significantly influences gene expression in lung alveolar epithelial cells. An increase in the expression of genes associated with autophagy, G2/M checkpoint and mTORC1 was observed, indicating cellular stress responses and adaptation to mechanical stimuli. Thus, this study not only showcases the AOC's potential to recapitulate alveolar epithelium mechanical cues but also offers a robust and scalable platform to be used for research and preclinical applications.