What question did this study set out to answer?

To establish an automated, rapid protocol for enumerating nucleated erythrocytes from digital images of stained thin blood smears to quantify hematozoan infection intensity.

August 1, 2004Journal of Parasitology

A Rapid Method for Counting Nucleated Erythrocytes on Stained Blood Smears by Digital Image Analysis

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Authors

EGEben GeringNova Southeastern University CACarter T. AtkinsonUnited States Geological Survey

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Implication

Methodological study demonstrates rapid automated counting of nucleated erythrocytes in nonmammalian blood smears, facilitating efficient quantification of hematozoan parasitemia.

Key Points

To establish an automated, rapid protocol for enumerating nucleated erythrocytes from digital images of stained thin blood smears to quantify hematozoan infection intensity.
Captured digital micrographs of Giemsa-stained thin blood smears from nonmammalian vertebrate hosts.
Applied the NIH-developed open-source software ImageJ to detect and count erythrocyte nuclei based on optical contrast, size, and morphological uniformity.
The protocol enabled rapid and accurate automated counting of total nucleated erythrocytes across extensive numbers of microscope fields.
The method provides a scalable approach for computing peripheral parasitemia ratios, particularly in cases of low-intensity intraerythrocytic infections.

Cite This Study

Gering et al. (2004) studied this question.

synapsesocial.com/papers/6a83525ef341e548e9d9d031 https://doi.org/10.1645/ge-222r

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Implication

Methodological study demonstrates rapid automated counting of nucleated erythrocytes in nonmammalian blood smears, facilitating efficient quantification of hematozoan parasitemia.

A Rapid Method for Counting Nucleated Erythrocytes on Stained Blood Smears by Digital Image Analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

A Rapid Method for Counting Nucleated Erythrocytes on Stained Blood Smears by Digital Image Analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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