Cytoplasmic granules were activated and moved along the cytoplasmic strands as the cultured muskmelon (Cucumis melo L.) cells were transferred from the k-8 agar medium to liquid medium. Cytochalasin B reversibly inhibited the streaming, suggesting that the motive force for streaming was generated by microfilament. Besides, KCN and 2,4-dinitrophenol lowered the streaming velocity, showing that the streaming required metabolic energy, ATP. Fluorescein diacetate (FDA) staining was used to estimate cell viability. Variety reticulatus (CR), which had been regarded as flooding untolerant (Crawford 1982, Liu and Chen 1987), had more viable cells than the flooding tolerant variety saccharinus (CS) when their calluses were transferred to liquid media. Meanwhile, variety CR had more streaming cells in the liquid medium, and was maintained steadily between 6th and 9th day after transfer. It is considered that cytoplasmic streaming occurring in the CR cells served as an adaptive mechanism under flooding stress.
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Chen et al. (1989) studied this question.