Plaque inhibition and distribution of chlorhexidine in Beagle dogs

Twelve Beagle dogs were allocated to three equal groups on the basis of existing plaque scores. Two gels, one containing 0.5 per cent chlorhexidine digluconate, the other a placebo, were applied once daily to the teeth of separate groups of animals, the third group serving as controls. The chlorhexidine gel inhibited the formation of plaque and reduced the severity of gingivitis. In a further study the distribution of 14 C‐labelled chlorhexidine was determined in the oral and systemic tissues of dogs after a single or repeated oral dose. Labelled material was observed in the superficial epithelial layers of all oral tissues; higher levels, of activity were present on keratinized than non‐keratinized surfaces. This process of adsorption was apparently reversible and no accumulation of chlorhexidine was detected in the mouth after repeated dosing. No absorption of labelled material through the oral tissues was observed.