What question did this study set out to answer?

To determine whether CagA and the vacuolating cytotoxin VacA are obligately coexpressed across clinical isolates of Helicobacter pylori and to define their phenotypic and genetic relationships.

January 1, 1995Infection and ImmunityOpen Access

Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin

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Authors

ZXZ XiangSecond Military Medical University SCStefano CensiniUniversity of Siena PBP F BayeliUniversity of Siena

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Implication

Laboratory analysis reveals two major bacterial phenotypes in Helicobacter pylori clinical isolates, indicating that CagA is not required for vacuolating cytotoxin expression.

Key Points

To determine whether CagA and the vacuolating cytotoxin VacA are obligately coexpressed across clinical isolates of Helicobacter pylori and to define their phenotypic and genetic relationships.
Evaluated genetic and phenotypic characteristics across 43 clinical isolates of Helicobacter pylori.
Assayed isolates for the presence of the CagA gene and the expression of both CagA protein and the vacuolating cytotoxin (VacA).
Identified type I strains (possessing the CagA gene and expressing both CagA and VacA) in 56% (24/43) of clinical isolates.
Identified type II strains (lacking the CagA gene and expressing neither factor) in 16% (7/43) of clinical isolates.
Observed an intermediate phenotype in 28% (12/43) of isolates, demonstrating that CagA is not required for the expression of the VacA cytotoxin.

Cite This Study

Xiang et al. (1995) studied this question.

synapsesocial.com/papers/6a835880b36623e7bea0f951 https://doi.org/10.1128/iai.63.1.94-98.1995

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Implication

Laboratory analysis reveals two major bacterial phenotypes in Helicobacter pylori clinical isolates, indicating that CagA is not required for vacuolating cytotoxin expression.

Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin

Discussion

Member takes

Implication

Key Points

Cite This Study

Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin

Discussion

Member takes

Implication

Key Points

Cite This Study

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