What question did this study set out to answer?

To investigate whether prostaglandin F2alpha and related prostaglandins can stimulate DNA synthesis and initiate cell proliferation in resting mouse fibroblasts.

July 1, 1975Proceedings of the National Academy of SciencesOpen Access

Initiation of cell proliferation in cultured mouse fibroblasts by prostaglandin F2alpha.

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Authors

LALuis Jiménez de AsúaThe Honourable Society of Lincoln's Inn DCDorothie ClinganMRC Clinical Trials Unit at UCL PRPhilip S. RudlandUniversity of Liverpool

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Implication

In vitro study demonstrates prostaglandin F2alpha triggers DNA synthesis in quiescent mouse fibroblasts, indicating prostaglandins act as extracellular growth regulators.

Key Points

To investigate whether prostaglandin F2alpha and related prostaglandins can stimulate DNA synthesis and initiate cell proliferation in resting mouse fibroblasts.
Exposed quiescent Swiss mouse 3T3 cell cultures to prostaglandin F2alpha, prostaglandin E1, or prostaglandin E2, with or without insulin.
Assessed initiation of DNA synthesis, cell division rates, and alterations in intracellular cyclic AMP and cyclic GMP levels.
Prostaglandin F2alpha initiated DNA synthesis and cell division in a minor proportion of quiescent Swiss 3T3 cells, showing higher potency than prostaglandins E1 and E2.
Insulin markedly potentiated the proliferative effect of prostaglandin F2alpha while concurrently decreasing cyclic AMP and increasing cyclic GMP concentrations.

Cite This Study

Asúa et al. (1975) studied this question.

synapsesocial.com/papers/6a835f43f091cb21d4ebf757 https://doi.org/10.1073/pnas.72.7.2724

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Implication

In vitro study demonstrates prostaglandin F2alpha triggers DNA synthesis in quiescent mouse fibroblasts, indicating prostaglandins act as extracellular growth regulators.

Initiation of cell proliferation in cultured mouse fibroblasts by prostaglandin F2alpha.

Discussion

Member takes

Implication

Key Points

Cite This Study

Initiation of cell proliferation in cultured mouse fibroblasts by prostaglandin F2alpha.

Discussion

Member takes

Implication

Key Points

Cite This Study

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