Sequencing, Cloning, and High-Level Expression of the pfp Gene, Encoding a PP i -Dependent Phosphofructokinase from the Extremely Thermophilic Eubacterium Dictyoglomus thermophilum

The sequencing, cloning, and expression of the pfp gene from Dictyoglomus thermophilum, which consists of 1,041 bp and encodes a pyrophosphate-dependent phosphofructokinase, are described. A phylogenetic analysis indicates that the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax. The recombinant and native enzymes share a high degree of similarity for most properties examined.