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Heat-sensitive prespores of Bacillus subtilis have been induced to germinate and outgrow while they are still enclosed within the mother cell. This intrasporangial germination has been obtained by transferring sporulating cells to rich medium after treatment with phenylmethylsulphonyl fluoride or toluene, or after subjecting them to cold-shock. The morphology of intrasporangial germination and outgrowth is described, and some possible explanations of the phenomenon are considered.
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Dion et al. (1978) studied this question.
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