What question did this study set out to answer?

Investigate the structural characteristics of cytoplasmic particles in nucleated cells and determine their association with aminoacyl transferase I activity.

April 1, 1970The Journal of Cell BiologyOpen Access

Cytoplasmic Particles and Aminoacyl Transferase I Activity

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Authors

ESEmma SheltonPurdue University West Lafayette EKEdward L. KuffNational Institutes of Health EMElizabeth S. MaxwellKing's College London

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Implication

Laboratory analysis reveals distinct 19S cytoplasmic particles co-sedimenting with aminoacyl transferase I in nucleated cells, suggesting a potential carrier particle role.

Key Points

Investigate the structural characteristics of cytoplasmic particles in nucleated cells and determine their association with aminoacyl transferase I activity.
Fractionated supernatant extracts from nucleated animal cells, plant cells, and Escherichia coli using sucrose gradient centrifugation.
Assayed gradient fractions for aminoacyl transferase I activity and imaged isolated structures via electron microscopy.
Aminoacyl transferase I activity resolved into two distinct sedimentation peaks at approximately 5–7S and 18–20S in eukaryotic extracts.
A rectangular particle measuring 100 x 145 Å sedimented at 19S, directly coinciding with the second peak of transferase I activity.
The rectangular particles were observed in supernatants from animal and plant cells but were absent in Escherichia coli extracts.

Cite This Study

Shelton et al. (1970) studied this question.

synapsesocial.com/papers/6a836cb29e2b23071beefe4d https://doi.org/10.1083/jcb.45.1.1

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Implication

Laboratory analysis reveals distinct 19S cytoplasmic particles co-sedimenting with aminoacyl transferase I in nucleated cells, suggesting a potential carrier particle role.

Cytoplasmic Particles and Aminoacyl Transferase I Activity

Discussion

Member takes

Implication

Key Points

Cite This Study

Cytoplasmic Particles and Aminoacyl Transferase I Activity

Discussion

Member takes

Implication

Key Points

Cite This Study

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