The intracellular distribution of free and potential phospholipases (EC 3.1.1.4) has been studied using isopycnic gradient centrifugation 06 rat pancreatic homogenates. 30 % of the free phopsholipase A 2 activity was found in the zymogen granules while only 15 % of the total phospholipase A activity was present there. Tryptic digestion increased the phospholipase A activity 20 fold‐an increase only in phospholipase A 2 activity. The zymogen granules accounted for 30 % of the prophospholipase and revealed a specific activity (activity per mg protein) 3 to 5 times higher than those of the other cell components. The intracellular distribution of prophospholipase is similar to lipase.
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B. Arnesjø (1971) studied this question.
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