What question did this study set out to answer?

Establish quantitative imaging protocols to measure cell membrane repair kinetics and subcellular trafficking events following localized injury.

March 24, 2014Journal of Visualized Experiments

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

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Establish quantitative imaging protocols to measure cell membrane repair kinetics and subcellular trafficking events following localized injury.
Developed an endpoint assay to simultaneously evaluate membrane repair capacity and lysosomal exocytosis across hundreds of injured adherent cells.
Applied pulsed laser micro-injury paired with real-time fluorescence imaging and TIRF microscopy to capture single-cell repair kinetics and vesicle dynamics at high spatial and temporal resolution.
Tested and validated protocols using cultured adherent muscle cells to monitor membrane repair and lysosomal trafficking.
Enabled real-time, quantitative tracking of membrane resealing kinetics at the single-cell level following localized pulsed-laser micro-injury.
Demonstrated reliable detection of lysosomal exocytosis during the resealing response across both multi-cell populations and single-vesicle TIRF imaging.
Provided adaptable imaging workflows applicable to diverse adherent cell types and varied subcellular compartments.

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Implication

Methodological study reveals quantitative imaging platforms for plasma membrane repair in cultured cells, highlighting mechanisms of subcellular trafficking.

Cite This Study

Defour et al. (2014) studied this question.

synapsesocial.com/papers/6a8375e852df268f62f70599 https://doi.org/10.3791/51106

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Implication

Methodological study reveals quantitative imaging platforms for plasma membrane repair in cultured cells, highlighting mechanisms of subcellular trafficking.

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

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Discussion

Implication

Cite This Study

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Key Points

Discussion

Implication

Cite This Study

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