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THE levels of 5,8,11-eicosatrienoate (triene) and arachidonate (tetraene) in animal tissues have been shown to be related to dietary linoleate (18:2). Holman (1960) first demonstrated that the ratio of triene:tetraene in the tissues of the rat is indicative of the essential fatty acid status of the animal. This criterion has been used by others to determine the 18:2 requirement of chicks. Hill (1966) estimated the 18:2 requirement of the male chick to be 2% of total dietary calories by plotting the ratio of triene:tetraene versus the percentage of total calories supplied by 18:2. Bieri and Prival (1966) determined the 18:2 requirement of male chicks on the basis of growth and the triene:tetraene ratio of fatty acids in liver and erythrocytes and found the requirement to be between 1–1.8%. Later, Hopkins and Nesheim (1967) estimated the requirement of straight-run chicks to be approximately 2.2 to 3.8% of total calories. Since…
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H. Menge (1970) studied this question.
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