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As Bracegirdle stated in History of Microtechnique “The first microscopists were preoccupied with making their microscopes and paid less attention to specimens for anything visible was impressive by its sheer novelty once attention was directed towards it by a lens.” The theory and practice of fixation and preservation of tissue specimens has always been a great topic of discussion. A brief history a fixation, fixative ingredients and techniques will help to unravel some of the “cross-links” of this vast topic. (The J Histotechnol 24:155, 2001)
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M. Lamar Jones (2001) studied this question.
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