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July 15, 1995Biochemical JournalOpen Access

Activation of the mitogen-activated protein kinase cascade by pertussis toxin-sensitive and -insensitive pathways in cultured ventricular cardiomyocytes

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Population

Ventricular cardiomyocytes cultured from neonatal rats

Comparison

Exposure to G-protein-coupled receptor agonists… vs Control cells or cells without PTX pretreatment

Design

Preclinical

Authors

MBMarie A. BogoyevitchElectrophysiologyACAngela ClerkHeart Failure / CardiomyopathyPSP H SugdenWaikato Hospital

Discussion

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Overview

Should not inform clinical GPCR targeting; leaves open adult human relevance of dual PTX-sensitive and -insensitive MAPK pathways.

Structured PICO

P
Population
Ventricular cardiomyocytes cultured from neonatal rats
I
Intervention
Exposure to G-protein-coupled receptor agonists (FCS, endothelin-1, phenylephrine, endothelin-3, lysophosphatidic acid, carbachol, isoprenaline, angiotensin II) with or without pertussis toxin (PTX) pretreatment
C
Comparator
Control (unstimulated) cells or cells without PTX pretreatment
O
Outcome
Activation of MAPK and MAPK kinase (MEK) activitysurrogate

Both PTX-sensitive (Gi/Go-linked) and PTX-insensitive (Gq/Gs-linked) pathways mediate MAPK activation in neonatal ventricular myocytes.

Cite This Study

Bogoyevitch et al. (1995) studied this question.

synapsesocial.com/papers/6a8382bd3965fa42e1c94e64https://doi.org/10.1042/bj3090437
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization of protein kinase C isotype expression in adult rat heart. Protein kinase C-epsilon is a major isotype present, and it is activated by phorbol esters, epinephrine, and endothelin.1993 · 249 citations
  2. 2The alpha 1A-adrenergic receptor subtype mediates biochemical, molecular, and morphologic features of cultured myocardial cell hypertrophy1993 · 301 citations
  3. 3A Divergence in the MAP Kinase Regulatory Network Defined by MEK Kinase and Raf1993 · 1,079 citations
  4. 4Pertussis toxin-sensitive activation of p21ras by G protein-coupled receptor agonists in fibroblasts.1993 · 378 citations