Predominant Distribution of Nifedipine-Insensitive, High Voltage–Activated Ca²⁺Channels in the Terminal Mesenteric Artery of Guinea Pig

We have found nifedipine-insensitive (NI), rapidly inactivating, voltage-dependent Ca2+ channels (current, NI-I(Ca)) with unique biophysical and pharmacological properties in the terminal branches of guinea pig mesenteric artery, by using a whole-cell mode of the patch-clamp technique. The fraction of NI-I(Ca) appeared to increase dramatically along the lower branches of mesenteric artery, amounting to almost 100% of global I(Ca) in its periphery. With 5 mmol/L Ba2+ as the charge carrier, NI-I(Ca) was activated with a threshold of -50 mV, peaked at -10 mV, and was half-activated and inactivated at -11 and -52 mV, respectively, generating a potential range of constant activation near the resting membrane potential. The NI-I(Ca) was rundown resistant, was not subject to Ca(2+)-dependent inactivation, and exhibited the pore properties typical for high voltage-activated Ca2+ channels; Ba2+ is approximately 2-fold more permeable than Ca2+, and Cd2+ is a better blocker than Ni2+ (IC(50), 6 and 68 micromol/L, respectively). Relatively specific blockers for N- and P/Q-type Ca2+ channels such as omega-conotoxins GVIA and MVIIC (each 1 micromol/L) and omega-agatoxin IVA (1 micromol/L) were ineffective at inhibiting NI-I(Ca), whereas nimodipine partially (10 micromol/L; approximately 40%) and amiloride potently ( approximately 75% with 1 mmol/L; IC(50); 107 micromol/L) blocked the current. Although these properties are reminiscent of R-type Ca2+ channels, expression of the alpha(1E) mRNA was not detected using reverse transcriptase-polymerase chain reaction. These results strongly suggest the predominant presence of NI, high voltage-activated Ca2+ channels with novel properties, which may be abundantly expressed in peripheral small arterioles and contribute to their tone regulation.