Hybridization, spheroplast (protoplast) fusion and transformation have been studied as means to genetically manipulate brewing and related yeast strains. Hybridization is a secondary technique for verifying the gene composition of recombinants produced by fusion and transformation. Further, it can be used to study gene dosage and gene suppression effects. Fusion is more empirical than manipulative since little control can be exerted over the genotypic makeup of the fusion product. Transformation with native DNA is a more subtle technique than fusion since the introduction of single traits into strains can be controlled. 2μm DNA plasmids are present in all brewing strains examined but the growth temperature of some strains is critical before significant numbers of plasmid can be detected. KEYWORDS Saccharomyces cerevisiae;, Sacch. uvarum (carls bergensis);, Kluyveromyces lactis;, hybridization, fusion, transformation, plasmid.
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Graham G. Stewart (1981) studied this question.
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