Solid-Phase Synthesis of Doubly Labeled Peptide Nucleic Acids as Probes for the Real-Time Detection of Hybridization

A substantial fluorescence enhancement was observed when novel peptide nucleic acid (PNA) probes were hybridized with complementary DNA in homogeneous solution. For the synthesis of the doubly labeled PNA probes, a highly flexible strategy was developed (see scheme). All reactions, including the labeling steps, were performed on the solid phase. Bhoc=benzhydryloxycarbonyl; Boc=tBuOCO.