What question did this study set out to answer?

To isolate and characterize the specific lactotransferrin receptor from mouse small intestinal brush-border membranes and determine its binding and structural features.

January 16, 1990Biochemistry

Isolation and partial characterization of a lactotransferrin receptor from mouse intestinal brush-border

View Full Paper

Ask AI

Bookmark

To isolate and characterize the specific lactotransferrin receptor from mouse small intestinal brush-border membranes and determine its binding and structural features.
Purified the membrane receptor to homogeneity using affinity chromatography on an immobilized human lactotransferrin column, followed by immunoaffinity chromatography using custom anti-receptor antibodies.
Characterized molecular mass, isoelectric point, and carbohydrate composition using SDS-PAGE, lectin binding assays, and enzymatic deglycosylation with N-glycanase and endoglycosidases.
Examined ligand binding affinity using iron-free and iron-saturated lactotransferrin, and cross-linked mouse lactotransferrin to its receptor using radiolabeled SASD.
The purified receptor is a single polypeptide chain with a relative molecular mass of approximately 130,000 and an isoelectric point of 5.8, binding both iron-free and iron-saturated lactotransferrin with a Kd of 0.1 µM.
Enzymatic cleavage with N-glycanase and endo-N-acetyl-beta-D-glucosaminidase B caused a molecular mass reduction of 25,000, while endo-H was ineffective, demonstrating that the receptor carries bi- and triantennary glycans.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Experimental study reveals the structural and binding properties of the intestinal lactotransferrin receptor in mice, highlighting mechanisms of lactotransferrin-mediated iron absorption.

Cite This Study

Hu et al. (1990) studied this question.

synapsesocial.com/papers/6a83cd42d65172829ee66180 https://doi.org/10.1021/bi00454a030

View Full Paper

Ask AI

Bookmark

Implication

Experimental study reveals the structural and binding properties of the intestinal lactotransferrin receptor in mice, highlighting mechanisms of lactotransferrin-mediated iron absorption.

Isolation and partial characterization of a lactotransferrin receptor from mouse intestinal brush-border

Key Points

Discussion

Implication

Cite This Study

Isolation and partial characterization of a lactotransferrin receptor from mouse intestinal brush-border

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider