What question did this study set out to answer?

To investigate the regulatory effects of specific polyamines on the key biosynthetic enzymes ornithine decarboxylase and S-adenosylmethionine decarboxylase using selective synthase inhibitors.

July 1, 1989Biochemical JournalOpen Access

Effects of S-adenosyl-1,8-diamino-3-thio-octane and S-methyl-5′-methylthioadenosine on polyamine synthesis in Ehrlich ascites-tumour cells

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Authors

IHIngvar HolmMalmö University LPLo PerssonLund University APAnthony E. PeggPennsylvania State University

Discussion

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Implication

Experimental study reveals complex feedback control of polyamine enzymes in Ehrlich ascites tumor cells, indicating multilevel regulation rather than direct structural suppression.

Key Points

To investigate the regulatory effects of specific polyamines on the key biosynthetic enzymes ornithine decarboxylase and S-adenosylmethionine decarboxylase using selective synthase inhibitors.
Cultured Ehrlich ascites-tumour cells were treated with the spermidine synthase inhibitor AdoDATO, the spermine synthase inhibitor MMTA, or both in combination.
Assayed cellular concentrations of putrescine, spermidine, and spermine alongside enzymatic activities and synthesis rates of ornithine decarboxylase and S-adenosylmethionine decarboxylase.
AdoDATO substantially reduced spermidine while increasing putrescine and spermine, without altering ornithine decarboxylase or S-adenosylmethionine decarboxylase activities.
MMTA markedly depleted spermine, elevated spermidine, and sharply increased S-adenosylmethionine decarboxylase activity via enzyme stabilization rather than polyamine depletion.
Combined inhibitor treatment transiently elevated ornithine decarboxylase activity via stimulated enzyme synthesis, while S-adenosylmethionine decarboxylase mRNA levels remained largely unchanged.

Cite This Study

Holm et al. (1989) studied this question.

synapsesocial.com/papers/6a83d1ed07bf35307a996dbe https://doi.org/10.1042/bj2610205

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July 1, 1989Biochemical JournalOpen Access

Effects of S-adenosyl-1,8-diamino-3-thio-octane and S-methyl-5′-methylthioadenosine on polyamine synthesis in Ehrlich ascites-tumour cells

View Full Paper

Ask AI

Bookmark

Authors

IHIngvar HolmMalmö University LPLo PerssonLund University APAnthony E. PeggPennsylvania State University

Discussion

Member takes

Implication

Experimental study reveals complex feedback control of polyamine enzymes in Ehrlich ascites tumor cells, indicating multilevel regulation rather than direct structural suppression.

Key Points

To investigate the regulatory effects of specific polyamines on the key biosynthetic enzymes ornithine decarboxylase and S-adenosylmethionine decarboxylase using selective synthase inhibitors.
Cultured Ehrlich ascites-tumour cells were treated with the spermidine synthase inhibitor AdoDATO, the spermine synthase inhibitor MMTA, or both in combination.
Assayed cellular concentrations of putrescine, spermidine, and spermine alongside enzymatic activities and synthesis rates of ornithine decarboxylase and S-adenosylmethionine decarboxylase.
AdoDATO substantially reduced spermidine while increasing putrescine and spermine, without altering ornithine decarboxylase or S-adenosylmethionine decarboxylase activities.
MMTA markedly depleted spermine, elevated spermidine, and sharply increased S-adenosylmethionine decarboxylase activity via enzyme stabilization rather than polyamine depletion.
Combined inhibitor treatment transiently elevated ornithine decarboxylase activity via stimulated enzyme synthesis, while S-adenosylmethionine decarboxylase mRNA levels remained largely unchanged.

Cite This Study

Holm et al. (1989) studied this question.

synapsesocial.com/papers/6a83d1ed07bf35307a996dbe https://doi.org/10.1042/bj2610205

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