Cytotoxicity analyses of Choukroun’s platelet-rich fibrin (PRF) on a wide range of human cells: The answer to a commercial controversy

Several important issues have been questioned following a reader’s mail concerning the type of tubes used to produce platelet-rich fibrin (PRF) and the possible cytotoxicity of silica-releasing tubes (glass-coated plastic tubes) for the recipient organism. In fact, numerous silica-based materials are used in dentistry, including as a direct bone-filling material. The present paper answers the questions step by step, then describes a cytotoxicity study carried on 4 different human cell types (gingival fibroblasts, keratinocytes, preadipocytes, and osteoblasts) placed in contact with PRF membranes for 12 hours, 24 hours, 3 days, and 7 days, respectively, of in vitro culture.