What question did this study set out to answer?

To investigate the inhibition of vacuolar H+-ATPases by bafilomycin A1 across macrophage membranes and evaluate its role in lysosomal enzyme exocytosis.

April 1, 1995Journal of Cellular Physiology

Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H+‐ATPase and induces lysosomal enzyme secretion in macrophages

View Full Paper

Ask AI

Bookmark

Authors

HTHans TapperLund University RSRoger SundlerMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

Member takes

Implication

Experimental study demonstrates vacuolar proton pump inhibition alters acidification and enzyme release in mouse macrophages, suggesting distinct subcellular regulation of H+-ATPases.

Key Points

To investigate the inhibition of vacuolar H+-ATPases by bafilomycin A1 across macrophage membranes and evaluate its role in lysosomal enzyme exocytosis.
Exposed mouse macrophages to varying concentrations of the vacuolar H+-ATPase inhibitor bafilomycin A1.
Assessed changes in lysosomal pH, phagosomal acidification rates, and the secretion of lysosomal enzymes.
Compared drug sensitivity profiles of H+-ATPases located in the plasma membrane, lysosomes, and phagosomes.
Bafilomycin A1 elevated lysosomal pH and triggered lysosomal enzyme release at similar drug concentrations.
Phagosomal acidification involved an initial bafilomycin A1-insensitive phase followed by a bafilomycin A1-sensitive proton transport mechanism.
Plasma membrane H+-ATPases showed higher sensitivity to bafilomycin A1 than lysosomal or phagosomal H+-ATPases, indicating distinct subcellular pump properties.

Cite This Study

Tapper et al. (1995) studied this question.

synapsesocial.com/papers/6a83dfbf448cdc0007ee849c https://doi.org/10.1002/jcp.1041630116

View Full Paper

Ask AI

Bookmark

Implication

Experimental study demonstrates vacuolar proton pump inhibition alters acidification and enzyme release in mouse macrophages, suggesting distinct subcellular regulation of H+-ATPases.

Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H+‐ATPase and induces lysosomal enzyme secretion in macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H+‐ATPase and induces lysosomal enzyme secretion in macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider