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Investigate the subcellular localization, isolation methodology, and macromolecular structure of particulate beta-glycogen in adult rabbit skeletal muscle.

July 1, 1968The Journal of Cell BiologyOpen Access

Rabbit Skeletal Muscle Glycogen

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Authors

JWJean‐Claude WansonUniversité Libre de Bruxelles PDP DrochmansUniversité Libre de Bruxelles

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Implication

Laboratory study reveals the structural assembly and sedimentation properties of particulate glycogen in rabbit skeletal muscle, indicating multiunit beta-particle architecture.

Key Points

Investigate the subcellular localization, isolation methodology, and macromolecular structure of particulate beta-glycogen in adult rabbit skeletal muscle.
Employed differential precipitation-centrifugation to isolate a glycogen-sarcovesicular fraction from adult skeletal muscle tissue.
Purified glycogen particles using deoxycholate washings and linear sucrose gradient centrifugation.
Analyzed particle morphology and molecular dynamics using negative-staining electron microscopy, analytical ultracentrifugation, and spectrophotometry of glycogen-iodine complexes.
Isolated glycogen contained 3% contaminating protein following deoxycholate treatment or linear sucrose gradient separation.
Negatively stained beta-particles demonstrated a mean diameter of 39.4 mμ, exhibiting conglomerate subunits of approximately 20 mμ that dissociated in concentrated potassium hydroxide.
Analytical ultracentrifugation showed sedimentation coefficients at infinite dilution between 115S and 135S, with iodine spectrophotometry revealing long outer chains.

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Wanson et al. (1968) studied this question.

synapsesocial.com/papers/6a83f2a5448cdc0007ee880b https://doi.org/10.1083/jcb.38.1.130

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Implication

Laboratory study reveals the structural assembly and sedimentation properties of particulate glycogen in rabbit skeletal muscle, indicating multiunit beta-particle architecture.

Rabbit Skeletal Muscle Glycogen

Discussion

Member takes

Implication

Key Points

Cite This Study

Rabbit Skeletal Muscle Glycogen

Discussion

Member takes

Implication

Key Points

Cite This Study

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