Techniques of signal assignment of 13 C‐NMR spectra of organic compounds, and in particular those of natural origin, are reviewed. They include symmetry, chemical shift considerations, spin‐spin coupling (both one‐bond and long‐range), spin relaxation (T 1 , NOE), isotopic enrichment and complexing agents. Special emphasis is given to the effects of oxygenated substituents on aromatic rings.
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Hugo E. Gottlieb (1977) studied this question.
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