Dynamics of Surface Modification in Myxovirus-Infected Cells

Research concerning the sites of synthesis, routes of transport, areas of accumulation, and ultimate distribution of viral specific protein in animal cells is currently the subject of extensive investigations utilizing techniques that provide an almost continuous spectrum of resolution. None of the methods reported, however, provides the resolution required to score individual virus particles simultaneously with a means for monitoring the entire surface of the living cell for newly acquired viral induced modifications. By careful application of the hemadsorption technique of Vogel and Shelokov (1957) to single myxovirus-infected cells, it is possible to obtain this degree of resolution under conditions that do not adversely affect viability of individual cells or preclude their isolation by standard cloning procedures. This report will describe the dynamic behavior of the surface of cells infected with myxovirus and will demonstrate the strikingly uniform pattern associated with the sequential events and movements occurring at the...