March 1, 1991AJP Cell Physiology
Alpha-adrenergic regulation of phosphoinositide metabolism and protein kinase C in isolated cardiac myocytes
Population
Isolated rat cardiac myocytes
Comparison
Norepinephrine in the presence of propranolol… vs Baseline/unstimulated state
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Alpha1-adrenergic PKC activation in myocytes is hypothesis-generating; human studies needed before clinical relevance.
Structured PICO
P
Population
Isolated rat cardiac myocytes
I
Intervention
Norepinephrine in the presence of propranolol (alpha 1-adrenergic stimulation), with or without depolarization (30 mM KCl) and increased extracellular Ca2+ (4 mM)
C
Comparator
Baseline/unstimulated state
O
Outcome
Levels of [3H]inositol phosphates and membrane-associated protein kinase Csurrogate
Alpha 1-adrenergic stimulation activates protein kinase C in cardiac myocytes, an effect amplified by elevated intracellular calcium through increased phosphoinositide metabolism.
Cite This Study
Kaku et al. (1991) studied this question.