The diisopropylphosphoryl- derivative of subtilisin BPN' was denatured and digested with trypsin. The tryptic digest was resolved on Dowex 50-X2, and 10 peptides were obtained in pure form. The sequences of these peptides account for 178 of the 280 residues of the protein. The amino-terminal and carboxyl-terminal peptides were identified.
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Kasper et al. (1966) studied this question.
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