What question did this study set out to answer?

To determine whether the human Duffy blood group antigen, the invasion receptor for Plasmodium vivax, is identical to the human erythrocyte chemokine receptor.

March 1, 1994Journal of Biological ChemistryOpen Access

Expression of the Duffy antigen in K562 cells. Evidence that it is the human erythrocyte chemokine receptor.

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Authors

ACAsok ChaudhuriNational Institutes of Health VZValerie ZbrzeznaNew York Blood Center JPJ PolyakovaNew York Blood Center

Discussion

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Implication

In vitro study demonstrates identical chemokine binding profiles for Duffy antigen and erythrocyte chemokine receptor in K562 cells, indicating they are the same protein.

Key Points

To determine whether the human Duffy blood group antigen, the invasion receptor for Plasmodium vivax, is identical to the human erythrocyte chemokine receptor.
Transfected the human Duffy antigen into the erythroleukemic cell line K562 to establish a stable expression system.
Conducted competition binding assays using 125I-labeled melanoma growth-stimulating activity (MGSA) against unlabeled chemokines (MGSA, IL-8, RANTES, MCP-1, PF4, MIP-1α, and MIP-1β) and the anti-Duffy monoclonal antibody Fy6.
Performed chemical cross-linking of 125I-MGSA to membrane proteins analyzed via SDS-polyacrylamide gel electrophoresis.
Transfected cells showed specific 125I-MGSA binding that was displaced by MGSA, IL-8, RANTES, MCP-1, and PF4, with dissociation constants (KD) of 24 ± 4.9 nM, 20 ± 4.7 nM, 41.9 ± 12.8 nM, and 33.9 ± 7 nM, respectively.
Binding was blocked by anti-Duffy monoclonal antibody Fy6, whereas MIP-1α and MIP-1β failed to displace 125I-MGSA binding.
Cross-linking covalently linked radiolabeled MGSA into a 47 kDa membrane protein, confirming that the Duffy antigen functions as the erythrocyte chemokine receptor.

Cite This Study

Chaudhuri et al. (1994) studied this question.

synapsesocial.com/papers/6a841b502327ba91ab8d709e https://doi.org/10.1016/s0021-9258(17)37123-5

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Implication

In vitro study demonstrates identical chemokine binding profiles for Duffy antigen and erythrocyte chemokine receptor in K562 cells, indicating they are the same protein.

Expression of the Duffy antigen in K562 cells. Evidence that it is the human erythrocyte chemokine receptor.

Discussion

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Implication

Key Points

Cite This Study

Expression of the Duffy antigen in K562 cells. Evidence that it is the human erythrocyte chemokine receptor.

Discussion

Member takes

Implication

Key Points

Cite This Study

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