Injector chambers for classical GC splitless injection must be large!

Injector overflow is determined quantitatively by analysis of the septum purge effluent. Vaporizing chambers must have an internal volume of about 1 ml ( e.g. 80 × 3.6 mm i.d.). This allows splitless injection of volumes between 1 and 2.5 μl, depending on the solvent. The septum purge should be closed during splitless transfer to prevent losses by back‐diffusion.