By taking advantage of the insensitivity of immobilized pH gradients to high protein and salt loads, microheterogeneity and polymorphism of α 1 ‐acid glycoprotein could be demonstrated on whole serum by conventional Coomassie Brilliant Blue G‐250 staining. Different running conditions were compared for optimal resolution.
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Gianazza et al. (1987) studied this question.
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