What question did this study set out to answer?

To purify and characterize the copper-containing dissimilatory nitrite reductase from the denitrifying bacterium Alcaligenes sp. NCIB 11015.

January 1, 1984The Journal of Biochemistry

Characterization of Nitrite Reductase from a Denitrifier, Alcaligenes Sp. NCIB 11015. A Novel Copper Protein1

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Authors

MMMasayuki MasukoSophia University HIHidekazu IwasakiNagoya University of Foreign Studies TSTakeshi SakuraiUniversity of Tsukuba

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Implication

Biochemical characterization reveals a functional nitrite reductase containing solely type I copper in Alcaligenes sp., highlighting a novel catalytic role for blue copper proteins.

Key Points

To purify and characterize the copper-containing dissimilatory nitrite reductase from the denitrifying bacterium Alcaligenes sp. NCIB 11015.
Purified the enzyme to homogeneity using CM-cellulose ion-exchange chromatography, Sephadex G-150 gel filtration, and hydroxyapatite adsorption.
Analyzed subunit structure and spectroscopic properties using SDS-PAGE, copper quantification, and electron paramagnetic resonance (EPR) spectroscopy.
Demonstrated that the enzyme is a homodimer of two 37,000 MW subunits with an isoelectric point of 8.4 and a redox potential of +260 mV at pH 7.2.
Identified two type I copper atoms per molecule with no other copper types detected, making it the first blue copper protein to display catalytic activity with only type I copper.
Observed a marked increase in enzymatic activity following freeze-thaw cycles at -20 °C.

Cite This Study

Masuko et al. (1984) studied this question.

synapsesocial.com/papers/6a8425517b4d6f59a47bf751 https://doi.org/10.1093/oxfordjournals.jbchem.a134856

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Implication

Biochemical characterization reveals a functional nitrite reductase containing solely type I copper in Alcaligenes sp., highlighting a novel catalytic role for blue copper proteins.

Characterization of Nitrite Reductase from a Denitrifier, Alcaligenes Sp. NCIB 11015. A Novel Copper Protein1

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Cite This Study

Characterization of Nitrite Reductase from a Denitrifier, Alcaligenes Sp. NCIB 11015. A Novel Copper Protein1

Discussion

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Implication

Key Points

Cite This Study

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