Overview of Protein Expression Vectors for E. coli

Escherichia coli is often the first host chosen for recombinant protein production, due to its low cost, ease of use, and the wide range of molecular tools available. After the gene of interest is cloned, the first task of the researcher is to choose a suitable protein expression vector for protein production. The number of possible vectors is vast, but a good background of the advantages and disadvantages of the various types of vectors can help in designing a successful strategy. This unit provides an introduction to the elements that make up an expression vector and guidance in applying those elements to the production of a particular protein.