What question did this study set out to answer?

To characterize the cyclic GMP-binding sites on bovine lung cyclic GMP-dependent protein kinase and assess their roles in kinase activation and autophosphorylation.

May 1, 1982Journal of Biological ChemistryOpen Access

Bovine lung cyclic GMP-dependent protein kinase exhibits two types of specific cyclic GMP-binding sites.

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Authors

CMCharles W. MackenzieUniversity of Nebraska Medical Center

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Implication

Biochemical analysis reveals two distinct cyclic GMP-binding sites in bovine lung kinase, indicating substrate-dependent regulation of enzymatic activity.

Key Points

To characterize the cyclic GMP-binding sites on bovine lung cyclic GMP-dependent protein kinase and assess their roles in kinase activation and autophosphorylation.
Measured cyclic GMP binding to bovine lung cyclic GMP-dependent protein kinase using cellulose ester filters with an ammonium sulfate wash buffer.
Evaluated enzyme autophosphorylation and histone kinase activity using arginine-rich histones and mixed histones across physiological cyclic GMP concentrations.
Identified two specific binding sites with physiological affinities (< 1 μM at 30 °C), including a rapidly dissociating Site II (half-life < 10 s at 0 or 30 °C).
Binding to high-affinity Site I selectively correlated with autophosphorylation and arginine-rich histone kinase stimulation, whereas both sites were required to stimulate kinase activity on mixed histones.

Cite This Study

Charles W. Mackenzie (1982) studied this question.

synapsesocial.com/papers/6a843d688bb79d42a483d60a https://doi.org/10.1016/s0021-9258(19)83818-8

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Implication

Biochemical analysis reveals two distinct cyclic GMP-binding sites in bovine lung kinase, indicating substrate-dependent regulation of enzymatic activity.

Bovine lung cyclic GMP-dependent protein kinase exhibits two types of specific cyclic GMP-binding sites.

Discussion

Member takes

Implication

Key Points

Cite This Study

Bovine lung cyclic GMP-dependent protein kinase exhibits two types of specific cyclic GMP-binding sites.

Discussion

Member takes

Implication

Key Points

Cite This Study

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