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Hamaguchi Y, Suzumura H, Taya M, Sakakura Y. ELISA for determination of immunoreactive free elastase and elastase in complex with α1-antritrypsin in nasal secretions with sinusitis. Acta Otolaryngol (Stockh) 1991; 111: 542-549.Sensitive double antibody sandwich ELISA methods was developed in order to quantify immunoreactive neutrophil elastase (NE) levels in nasal secretions with chronic sinusitis (CS). Microwell plate as a solid phase was coated with anti-NE antibody. Two different horseradish peroxidase (HRP) labelled antibodies used as the second antibody were anti-NE-HRP for measuring total (free+complexed) NE level and anti-α1-antitrypsin (AT)-HRP for complexed NE level. Mean value of total NE was 31.0+20.7 ug/ml in nasal secretions from adult patients with CS, and the percentage of complexed NE in total NE was 33.7+21.4%. This sandwich ELISA is a useful method for measuring both total and complexed NE levels in nasal secretions.
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Hamaguchi et al. (1991) studied this question.
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