Key result
Serotonin transporter deficiency significantly reduced venous thrombus volume compared to wild-type mice (1.09 vs 13.1 mm3), demonstrating that thrombosis extent depends on the serotonin transporter.
Why the study?
Because deep vein thrombosis induces thromboinflammation and serotonin has an immunomodulatory role in inflammation, this study examined the impact of peripheral platelet serotonin on venous thrombosis development.
Does serotonin transporter deficiency or absence of platelet serotonin impair thrombus formation in a mouse model of deep vein thrombosis?
Does serotonin transporter deficiency or absence of platelet serotonin impair thrombus formation in a mouse model of deep vein thrombosis?
Absolute Event Rate: 1.09% vs 13.1%
p-value: p=0.002
Serotonin transporter deficiency, but not the mere absence of peripheral serotonin, significantly reduces venous thrombus formation and neutrophil recruitment in a mouse model of deep vein thrombosis.
SERT deficiency attenuates murine DVT; hypothesis-generating for serotonin transporter targeting in human venous thromboembolism prevention.
Background: The majority of peripheral serotonin is stored in dense granules of circulating blood platelets and released upon platelet activation. Recently, an immunomodulatory role of serotonin in inflammation has been found, influencing the recruitment of leukocytes, especially neutrophils. Objectives: Since deep vein thrombosis creates an inflammatory milieu, called thromboinflammation, this study examined the impact of peripheral platelet serotonin on the development of venous thrombosis. Methods: ) mice were used. Thrombus volume was measured, and its composition was analyzed after 48 hours using immunofluorescence microscopy. Neutrophils and platelet-neutrophil complexes were analyzed using flow cytometry. Results: mice, there was no decrease in circulating platelet-neutrophil complexes. Conclusion: The extent of venous thrombosis did not depend on peripheral serotonin in our mouse model, but rather on the presence of the serotonin transporter. In the absence of the serotonin transporter, the thrombogenic property as well as the overall immune response to venous thrombosis was reduced. The distribution of the serotonin transporter on immune cells and its thrombogenic potential should be studied further.
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Naber et al. (2025) studied Deep vein thrombosis. Serotonin transporter (SERT) deficiency vs. Wild-type (WT) mice was evaluated on Thrombus volume (p=0.002). Serotonin transporter deficiency significantly reduced venous thrombus volume compared to wild-type mice (1.09 vs 13.1 mm3), demonstrating that thrombosis extent depends on the serotonin transporter.
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