What question did this study set out to answer?

To isolate and characterize the biochemical, structural, and oxygen-binding properties of hemoglobin from the parasitic flatworm Dicrocoelium dendriticum.

August 1, 1978European Journal of BiochemistryOpen Access

Isolation and Characterization of the Hemoglobin from the Lanceolate Fluke Dicrocoelium dendriticum

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Authors

PTP. TuchschmidUniversity of Zurich PKPeter A. KunzUniversity of Zurich KWKenneth J. WilsonNational Institutes of Health

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Implication

Biochemical analysis reveals high oxygen affinity in hemoglobin from Dicrocoelium dendriticum, indicating evolutionary adaptation to hypoxic host bile ducts.

Key Points

To isolate and characterize the biochemical, structural, and oxygen-binding properties of hemoglobin from the parasitic flatworm Dicrocoelium dendriticum.
Purified the parasite's hemoglobin using gel filtration and ion-exchange chromatography.
Determined molecular weight under native and denaturing conditions, analyzed ligand derivatives, and measured oxygen-binding affinity (p50 and Hill coefficient nH).
Characterized terminal sequences and isoform charge distribution using dansylation, carboxypeptidase A digestion, and isoelectric focusing.
Denatured hemoglobin exhibited a subunit molecular weight of 15,500 Da (apparent native molecular weight of 22,000 Da in 0.01 M phosphate buffer, pH 7.4) and non-covalently bound one ferroprotoporphyrin IX per chain.
Displayed high oxygen affinity with a p50 of 0.07 to 0.1 mmHg (9.3 to 13.3 Pa at 20 °C, pH 7.0) and non-cooperative binding (Hill coefficient nH = 1.0).
Identified two native isoforms with isoelectric points of 4.51 and 4.53 sharing identical amino-acid compositions, an alanine amino-terminus, and a -His-Ala-Leu carboxy-terminal sequence.

Cite This Study

Tuchschmid et al. (1978) studied this question.

synapsesocial.com/papers/6a844dab6e9d0d88c0bd9d64 https://doi.org/10.1111/j.1432-1033.1978.tb12460.x

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Implication

Biochemical analysis reveals high oxygen affinity in hemoglobin from Dicrocoelium dendriticum, indicating evolutionary adaptation to hypoxic host bile ducts.

Isolation and Characterization of the Hemoglobin from the Lanceolate Fluke Dicrocoelium dendriticum

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and Characterization of the Hemoglobin from the Lanceolate Fluke Dicrocoelium dendriticum

Discussion

Member takes

Implication

Key Points

Cite This Study

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