Efficiency Comparison of a Novel E2 Subunit Vaccine and a Classic C-Strain Vaccine against Classical Swine Fever

Figures

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  Figure 1. Diagram of the animal experimental design. Piglets received an initial intramuscular injection that was defined as occurring at 0 days post vaccination (dpv).

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  Figure 2. The survival curves, body temperatures, viremia, and virus shedding of the experimental pigs. (A) Survival rate, the lines of dates are covered by the lines of DMEM and CSFV data. (B) Body temperature. (C) Viral copies in oropharyngeal swab samples. (D) Viral copies in the blood. The dotted line represents the minimum detection threshold. DMEM: the blank control group that received no immunization and no challenge. CSFV: the infection control group that received no immunization. C-vac one dose and E2-vac one dose: pigs were intramuscularly injected with the relevant vaccines at 23 dpv. C-vac two doses and E2-vac two doses: pigs were intramuscularly injected with the relevant vaccines at 0 dpv and received a booster immunization at 23 dpv. At 35 dpv, pigs in all groups except DMEM group were challenged with ASFV.

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  Figure 3. Antibody responses in pigs in the two-dose (A,B) and one-dose (C,D) vaccination groups according to the blocking ELISAs. Gray columns represent the ELISA blocking rate of the C-vac groups, and white columns represent the ELISA blocking rate of the E2-vac groups. The dotted line represents a positive threshold: E2 > 0.4, E0 > 0.5, NS3 > 0.25.