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To characterize the mechanism by which bacterial CRISPR-Cas systems employ the Cas9 endonuclease to neutralize invading genetic elements.

Protein and Nucleic Acid Sequence Database Systems

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To characterize the mechanism by which bacterial CRISPR-Cas systems employ the Cas9 endonuclease to neutralize invading genetic elements.
Evaluated bacterial clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) machinery.
Analyzed dual RNA-guided Cas9 endonuclease activity targeting invading bacteriophages and conjugative plasmids.
Demonstrated that Cas9 functions as a dual RNA-guided DNA endonuclease in bacterial adaptive immunity.
Identified that Cas9 introduces site-specific double-strand breaks to neutralize foreign mobile genetic elements.

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Molecular analysis reveals RNA-guided Cas9 endonuclease defense in bacteria, highlighting mechanisms of targeted nucleic acid cleavage.

ORCUTT et al. (1983) studied this question.

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