Population
Escherichia coli expressing human rhinovirus type 14 protease 3C
Comparison
Expression and purification using… vs Mutant protease produced and purified in the…
Design
Preclinical
Authors
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Enables scalable production of functional viral protease for inhibitor screens; leaves open any clinical translation.
Human rhinovirus type 14 protease 3C can be successfully expressed and purified in E. coli, retaining its specific cleavage activity, which is abolished by a Cys146 to Ser mutation.
KNOTT et al. (1989) studied this question.
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